Deletion of Swm2p selectively impairs trimethylation of snRNAs by trimethylguanosine synthase (Tgs1p).
The 5' cap trimethylation of small nuclear (snRNAs) and several nucleolar RNAs (snoRNAs) by trimethylguanosine synthase 1 (Tgs1p) is required for efficient pre-mRNA splicing. The previously uncharacterised protein Swm2p interacted with Tgs1p in yeast two-hybrid screens. In the present study we show that Swm2p interacts with the N-terminus of Tgs1p ... and its deletion impairs pre-mRNA splicing and pre-rRNA processing. The trimethylation of spliceosomal snRNAs and the U3 snoRNA, but not other snoRNAs, was abolished in the absence of Swm2p, indicating that Swm2p is required for a substrate-specific activity of Tgs1p.
Mesh Terms:
Carrier Proteins, Cell Nucleolus, Gene Deletion, Methylation, Methyltransferases, Protein Binding, RNA 3' End Processing, RNA Precursors, RNA Splicing, RNA, Small Nuclear, RNA, Small Nucleolar, Ribosome Subunits, Small, Eukaryotic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity
Carrier Proteins, Cell Nucleolus, Gene Deletion, Methylation, Methyltransferases, Protein Binding, RNA 3' End Processing, RNA Precursors, RNA Splicing, RNA, Small Nuclear, RNA, Small Nucleolar, Ribosome Subunits, Small, Eukaryotic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity
FEBS Lett.
Date: Aug. 04, 2010
PubMed ID: 20621096
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