Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA.
In Saccharomyces cerevisiae, in vitro mRNA cleavage and polyadenylation require the poly(A) binding protein, Pab1p, and two multiprotein complexes: CFI (cleavage factor I) and CPF (cleavage and polyadenylation factor). We characterized a novel essential gene, MPE1 (YKL059c), which interacts genetically with the PCF11 gene encoding a subunit of CFI. Mpe1p ... is an evolutionarily conserved protein, a homolog of which is encoded by the human genome. The protein sequence contains a putative RNA-binding zinc knuckle motif. MPE1 is implicated in the choice of ACT1 mRNA polyadenylation site in vivo. Extracts from a conditional mutant, mpe1-1, or from a wild-type extract immunoneutralized for Mpe1p are defective in 3'-end processing. We used the tandem affinity purification (TAP) method on strains TAP-tagged for Mpe1p or Pfs2p to show that Mpe1p, like Pfs2p, is an integral subunit of CPF. Nevertheless a stable CPF, devoid of Mpe1p, was purified from the mpe1-1 mutant strain, showing that Mpe1p is not directly involved in the stability of this complex. Consistently, Mpe1p is also not necessary for the processive polyadenylation, nonspecific for the genuine pre-mRNA 3' end, displayed by the CPF alone. However, a reconstituted assay with purified CFI, CPF, and the recombinant Pab1p showed that Mpe1p is strictly required for the specific cleavage and polyadenylation of pre-mRNA. These results show that Mpe1p plays a crucial role in 3' end formation probably by promoting the specific link between the CFI/CPF complex and pre-mRNA.
Mesh Terms:
Alleles, Amino Acid Sequence, Blotting, Northern, Conserved Sequence, Electrophoresis, Polyacrylamide Gel, Evolution, Molecular, Immunoblotting, Mass Spectrometry, Molecular Sequence Data, Polyadenylation, Protein Binding, RNA, RNA, Messenger, RNA-Binding Proteins, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Temperature, Time Factors, Zinc, mRNA Cleavage and Polyadenylation Factors
Alleles, Amino Acid Sequence, Blotting, Northern, Conserved Sequence, Electrophoresis, Polyacrylamide Gel, Evolution, Molecular, Immunoblotting, Mass Spectrometry, Molecular Sequence Data, Polyadenylation, Protein Binding, RNA, RNA, Messenger, RNA-Binding Proteins, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Temperature, Time Factors, Zinc, mRNA Cleavage and Polyadenylation Factors
Mol. Cell. Biol.
Date: Dec. 01, 2001
PubMed ID: 11713271
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