beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation.
beta-Arrestins were initially shown, in conjunction with G protein-coupled receptor kinases, to be involved in the desensitization and internalization of activated seven-transmembrane receptors. Recently, beta-arrestin 2 has been shown to act as a signal mediator in mitogen-activated protein kinase cascades and to play a positive regulatory role in chemotaxis. We ... now show that beta-arrestin 1 is required to activate the small GTPase RhoA leading to the re-organization of stress fibers following the activation of the angiotensin II type 1A receptor. This angiotensin II type 1A receptor-directed RhoA activation and stress fiber formation also require the activation of the heterotrimeric G protein G(alphaq/11). Whereas neither beta-arrestin 1 nor G(alphaq/11) activation alone is sufficient to robustly activate RhoA, the concurrent recruitment of beta-arrestin 1 and activation of G(alphaq/11) leads to full activation of RhoA and to the subsequent formation of stress fibers.
Mesh Terms:
Actins, Arrestins, Cell Line, Chemotaxis, Cross-Linking Reagents, Cytoskeleton, Dimerization, Electrophoresis, Polyacrylamide Gel, Endocytosis, Enzyme Activation, GTP-Binding Protein alpha Subunits, Gq-G11, Glutathione Transferase, Green Fluorescent Proteins, Humans, Immunoblotting, Immunoprecipitation, Inositol Phosphates, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Models, Biological, Protein Binding, RNA Interference, RNA, Small Interfering, Receptor, Angiotensin, Type 1, Recombinant Fusion Proteins, Signal Transduction, Time Factors, Transfection, rhoA GTP-Binding Protein
Actins, Arrestins, Cell Line, Chemotaxis, Cross-Linking Reagents, Cytoskeleton, Dimerization, Electrophoresis, Polyacrylamide Gel, Endocytosis, Enzyme Activation, GTP-Binding Protein alpha Subunits, Gq-G11, Glutathione Transferase, Green Fluorescent Proteins, Humans, Immunoblotting, Immunoprecipitation, Inositol Phosphates, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Models, Biological, Protein Binding, RNA Interference, RNA, Small Interfering, Receptor, Angiotensin, Type 1, Recombinant Fusion Proteins, Signal Transduction, Time Factors, Transfection, rhoA GTP-Binding Protein
J. Biol. Chem.
Date: Mar. 04, 2005
PubMed ID: 15611106
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