Saccharomyces cerevisiae 14-3-3 proteins Bmh1 and Bmh2 participate in the process of catabolite inactivation of maltose permease.
In this study we show that Reg1, the regulatory subunit of the Reg1/Glc7 protein phosphatase (PP1) complex, interacts physically with the two yeast members of the 14-3-3 protein family, Bmh1 and Bmh2. By using different fragments of the Reg1 protein we mapped the interaction domain at the N-terminal part of ... the protein. We also show that Reg1 and yeast 14-3-3 proteins participate actively in the regulation of the glucose-induced degradation of maltose permease (Mal61).
Mesh Terms:
14-3-3 Proteins, Enzyme Inhibitors, Immunoblotting, Membrane Transport Modulators, Membrane Transport Proteins, Models, Genetic, Monosaccharide Transport Proteins, Oligonucleotides, Protein Binding, Protein Structure, Tertiary, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Symporters, Temperature, Time Factors, Two-Hybrid System Techniques, Tyrosine 3-Monooxygenase, beta-Galactosidase
14-3-3 Proteins, Enzyme Inhibitors, Immunoblotting, Membrane Transport Modulators, Membrane Transport Proteins, Models, Genetic, Monosaccharide Transport Proteins, Oligonucleotides, Protein Binding, Protein Structure, Tertiary, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Symporters, Temperature, Time Factors, Two-Hybrid System Techniques, Tyrosine 3-Monooxygenase, beta-Galactosidase
FEBS Lett.
Date: Jun. 05, 2003
PubMed ID: 12782308
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