Malloy MT, McIntosh DJ, Walters TS, Flores A, Goodwin JS, Arinze IJ

Ubiquitylation of Nrf2 by the Keap1-Cullin3/RING box1 (Cul3-Rbx1) E3 ubiquitin ligase complex targets Nrf2 for proteasomal degradation in the cytoplasm and is an extensively studied mechanism for regulating the cellular level of Nrf2. Although mechanistic details are lacking, reports abound that Nrf2 can also be degraded in the nucleus. Here, ...

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we demonstrate that Nrf2 is a target for sumoylation by both SUMO-1 and SUMO-2 in the intact cell. An in vitro sumoylation assay confirmed that Nrf2 is a substrate for conjugation with SUMO-1 as well as with SUMO-2. HepG2 cells treated with As2O3, which enhances attachment of SUMO-2/3 to target proteins, increased SUMO-2/3-modification (poly-sumoylation) of Nrf2. We show that Nrf2 traffics, in part, to promyelocytic leukemia-nuclear bodies (PML-NBs). Cell fractions harboring key components of PML-NBs did not contain biologically active Keap1, but contained modified Nrf2 as well as RING finger protein 4 (RNF4), a poly-SUMO-specific E3 ubiquitin ligase. Overexpression of wild-type RNF4, but not the catalytically inactive mutant, decreased the steady-state levels of Nrf2, measured in the PML-NB-enriched cell fraction. The proteasome inhibitor MG-132 interfered with this decrease, resulting in elevated levels of poly-sumoylated Nrf2 that was also ubiquitylated. Wild-type RNF4 accelerated the half life (t1/2) of Nrf2, measured in PML-NB-enriched cell fraction. These results suggest that RNF4 mediates poly-ubiquitylation of poly-sumoylated Nrf2, leading to its subsequent degradation in PML-NBs. Overall, this work identifies Nrf2 as a target for sumoylation and provides a novel mechanism for its degradation in the nucleus, independent of Keap1.

Date: Mar. 29, 2013

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