Insights into the structure of the CCR4-NOT complex by electron microscopy.

The CCR4-NOT complex is a deadenylation complex, which plays a major role for mRNA stability. The complex is conserved from yeast to human and consists of nine proteins NOT1-NOT5, CCR4, CAF1, CAF40 and CAF130. We have successfully isolated the complex using a Protein A tag on NOT1, followed by cross-linking ...
on a glycerol gradient. All components of the complex were identified by mass spectrometry. Electron microscopy of negatively stained particles followed by image reconstruction revealed an L-shaped complex with two arms of similar length. The arms form an accessible cavity, which we think could provide an extensive interface for RNA-deadenylation.
Mesh Terms:
Cell Cycle Proteins, Humans, Mass Spectrometry, Microscopy, Electron, Models, Molecular, Multiprotein Complexes, Protein Subunits, RNA, Messenger, Ribonucleases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
FEBS Lett.
Date: Jul. 21, 2011
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