Self-assembly of minimal COPII cages.
The small G-protein Sar1 and the cytosolic complexes Sec23/24 and Sec13/31 associate sequentially on endoplasmic reticulum membranes to form a protein coat named COPII, which drives the formation of transport vesicles. Using dynamic light scattering, we show that Sec23/24 and Sec13/31 can self-assemble in a stoichiometric manner in solution to ... form particles with hydrodynamic radii in the range of 40-60 nm. Self-assembly is favoured by lowering the pH, the ionic strength and/or the temperature. Electron microscopy reveals the formation of spherical particles 60-120 nm in diameter with a tight, rough mesh on their surfaces. We suggest that these structures, which represent a minimal COPII cage, mimic the molecular organization of the membrane-associated COPII coat.
Mesh Terms:
COP-Coated Vesicles, Carrier Proteins, Clathrin, GTPase-Activating Proteins, Kinetics, Light, Membrane Proteins, Microscopy, Electron, Nuclear Pore Complex Proteins, Phospholipid Transfer Proteins, Saccharomyces cerevisiae Proteins, Scattering, Radiation
COP-Coated Vesicles, Carrier Proteins, Clathrin, GTPase-Activating Proteins, Kinetics, Light, Membrane Proteins, Microscopy, Electron, Nuclear Pore Complex Proteins, Phospholipid Transfer Proteins, Saccharomyces cerevisiae Proteins, Scattering, Radiation
EMBO Rep.
Date: Apr. 01, 2003
PubMed ID: 12671686
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