Kcc4 associates with septin proteins of Saccharomyces cerevisiae.
Kcc4, a kinase of the budding yeast Saccharomyces cerevisiae, is homologous to the bud neck protein kinases Hsl1/Nik1 and Gin4. We report here that a GFP-Kcc4 fusion protein is localized at the bud neck and that the non-kinase domain is required for this localization. We also demonstrate that Kcc4 associates ... with septin proteins in vitro and in vivo by two-hybrid analysis, GST pull-down experiments, immunoprecipitation, and analysis of direct association with affinity-purified GST-Kcc4 and MBP-Septin proteins. From the results obtained here, we suggest that Cdc11 is the primary association partner of Kcc4.
Mesh Terms:
Cell Cycle Proteins, Cytoskeletal Proteins, Fungal Proteins, Glutathione Transferase, Green Fluorescent Proteins, Immunoblotting, Luminescent Proteins, Microscopy, Fluorescence, Protein Binding, Protein Kinases, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Two-Hybrid System Techniques
Cell Cycle Proteins, Cytoskeletal Proteins, Fungal Proteins, Glutathione Transferase, Green Fluorescent Proteins, Immunoblotting, Luminescent Proteins, Microscopy, Fluorescence, Protein Binding, Protein Kinases, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Two-Hybrid System Techniques
FEBS Lett.
Date: Feb. 02, 2001
PubMed ID: 11165249
View in: Pubmed Google Scholar
Download Curated Data For This Publication
15556
Switch View:
- Interactions 6