Cdc28 activates exit from mitosis in budding yeast.

The activity of the cyclin-dependent kinase 1 (Cdk1), Cdc28, inhibits the transition from anaphase to G1 in budding yeast. CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive to perturbations that arrest cells in mitosis. Surprisingly, this behavior is not due ...
to a lack of inhibitory phosphorylation or increased kinase activity, but reflects reduced activity of the anaphase-promoting complex (APC), a defect shared with other mutants that lower Cdc28/Clb activity in mitosis. CDC28-VF has reduced Cdc20- dependent APC activity in mitosis, but normal Hct1- dependent APC activity in the G1 phase of the cell cycle. The defect in Cdc20-dependent APC activity in CDC28-VF correlates with reduced association of Cdc20 with the APC. The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast.
Mesh Terms:
Anaphase, CDC2-CDC28 Kinases, Cell Cycle Proteins, Fungal Proteins, G1 Phase, Genes, cdc, Mitosis, Mitotic Spindle Apparatus, Mutation, Phosphorylation, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Saccharomycetales, Time Factors
J. Cell Biol.
Date: Jun. 26, 2000
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