Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.
Cell cycle arrest of Saccharomyces cerevisiae in G1 by the antimitogen alpha-factor is mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Far1 protein is required for cell cycle arrest and associates with the Cdc28-Cln complex. The kinase activity of Cdc28-Cln ... was directly inhibited by Far1 both in vivo and in vitro, thus demonstrating that Far1 acts at the final step in the alpha-factor response pathway by inhibiting a G1 cyclin-dependent kinase.
Mesh Terms:
CDC28 Protein Kinase, S cerevisiae, Cell Cycle Proteins, Cyclin-Dependent Kinase Inhibitor Proteins, Cyclins, Fungal Proteins, G1 Phase, Peptides, Phosphorylation, Protamine Kinase, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
CDC28 Protein Kinase, S cerevisiae, Cell Cycle Proteins, Cyclin-Dependent Kinase Inhibitor Proteins, Cyclins, Fungal Proteins, G1 Phase, Peptides, Phosphorylation, Protamine Kinase, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Science
Date: Aug. 26, 1994
PubMed ID: 8066461
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