Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast.
Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgtlp is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele-specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich ... repeat domain of the adenylyl cyclase Cyrlp/Cdc35p. We demonstrate that Sgt1p and Cyrlp/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgtlp has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Adenylate Cyclase, Alleles, Amino Acid Sequence, Base Sequence, Cyclic AMP, DNA, Fungal, Enzyme Activation, Genes, Fungal, Models, Molecular, Molecular Chaperones, Molecular Sequence Data, Mutation, Protein Structure, Tertiary, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Temperature
Adaptor Proteins, Signal Transducing, Adenylate Cyclase, Alleles, Amino Acid Sequence, Base Sequence, Cyclic AMP, DNA, Fungal, Enzyme Activation, Genes, Fungal, Models, Molecular, Molecular Chaperones, Molecular Sequence Data, Mutation, Protein Structure, Tertiary, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Temperature
Eukaryotic Cell
Date: Aug. 01, 2002
PubMed ID: 12456005
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