Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.
The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also ... includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCFCdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G1 to S cell cycle transition. These findings provide a further link between VHL and the cellular ubiquitination machinery.
Mesh Terms:
Amino Acid Sequence, Animals, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cell Line, Cullin Proteins, Cyclin-Dependent Kinase Inhibitor Proteins, F-Box Proteins, Fungal Proteins, Ligases, Liver, Male, Molecular Sequence Data, Peptide Synthases, Proteins, Rats, Rats, Sprague-Dawley, Recombinant Fusion Proteins, S-Phase Kinase-Associated Proteins, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitins, Von Hippel-Lindau Tumor Suppressor Protein
Amino Acid Sequence, Animals, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cell Line, Cullin Proteins, Cyclin-Dependent Kinase Inhibitor Proteins, F-Box Proteins, Fungal Proteins, Ligases, Liver, Male, Molecular Sequence Data, Peptide Synthases, Proteins, Rats, Rats, Sprague-Dawley, Recombinant Fusion Proteins, S-Phase Kinase-Associated Proteins, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitins, Von Hippel-Lindau Tumor Suppressor Protein
Science
Date: Apr. 23, 1999
PubMed ID: 10213691
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