Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin.

Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the ...
prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication. During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.
Mesh Terms:
CDC28 Protein Kinase, S cerevisiae, Carrier Proteins, Cell Cycle Proteins, Chromatin, Chromosomal Proteins, Non-Histone, Cyclin-Dependent Kinases, Cyclins, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Enzyme Activation, Fungal Proteins, G1 Phase, Nuclear Proteins, Origin Recognition Complex, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Science
Date: Apr. 24, 1998
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