Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4.
In eukaryotes, the ubiquitin-proteasome system plays a major role in selective protein breakdown for cellular regulation. Here we report the discovery of a new essential component of this degradation machinery. We found the Saccharomyces cerevisiae protein Cic1 attached to 26S proteasomes playing a crucial role in substrate specificity for proteasomal ... destruction. Whereas degradation of short-lived test proteins is not affected, cic1 mutants stabilize the F-box proteins Cdc4 and Grr1, substrate recognition subunits of the SCF complex. Cic1 interacts in vitro and in vivo with Cdc4, suggesting a function as a new kind of substrate recruiting factor or adaptor associated with the proteasome.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Cell Cycle Proteins, F-Box Proteins, Fungal Proteins, Humans, Molecular Sequence Data, Peptide Hydrolases, Peptide Synthases, Proteasome Endopeptidase Complex, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae Proteins, Substrate Specificity, Ubiquitin-Protein Ligases, Ubiquitins
Amino Acid Sequence, Carrier Proteins, Cell Cycle Proteins, F-Box Proteins, Fungal Proteins, Humans, Molecular Sequence Data, Peptide Hydrolases, Peptide Synthases, Proteasome Endopeptidase Complex, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae Proteins, Substrate Specificity, Ubiquitin-Protein Ligases, Ubiquitins
EMBO J.
Date: Aug. 15, 2001
PubMed ID: 11500370
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