Correct assembly of the RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae.

Recent papers have provided insight into the cytoplasmic assembly of the RNA polymerase II and its transport to the nucleus. However, little is known about the mechanisms governing its nuclear assembly, stability, degradation, and recycling. We demonstrate that the foot of the RNA pol II is crucial for the assembly ...
and stability of the complex, by ensuring the correct association of Rpb1-Rpb6, and of the dimer Rpb4/7. Mutations at the foot affect the assembly and stability of the enzyme, a defect that is offset by RPB6 overexpression, coordinated with Rpb1 degradation by an Asr1-independent mechanisms. Correct assembly is a prerequisite to properly maintain several transcription steps. In fact, assembly defects alter transcriptional activity, the amount of enzyme associated with the genes, affects the CTD phosphorylation, interferes with mRNA capping machinery, and suggest an increase in stalled RNA pol II. In addition, our data show that TBP occupancy does not correlate with RNA pol II occupancy or transcriptional activity, suggesting a functional relationship between assembly, Mediator and PIC stability. Finally, our data help clarify the mechanisms governing assembly and stability of the RNA pol II.
Mol. Cell. Biol.
Date: Jul. 08, 2013
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