Tripodi F, Nicastro R, Busnelli S, Cirulli C, Maffioli E, Tedeschi G, Alberghina L, Coccetti P

In Saccharomyces cerevisiae, the entrance into S phase requires the activation of a specific burst of transcription, which depends on SBF (Swi4-Swi6) and MBF (Mbp1-Swi6) complexes. CK2 is a pleiotropic kinase, involved in several cellular processes including the regulation of cell cycle. CK2 is composed of two catalytic subunits (α ...

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and α') and two regulatory subunits (β and β'), both of which are required to form the active holoenzyme.Here we investigate the function of the CK2 holoenzyme in Start-specific transcription. The mutant strain ckb1Δckb2Δ, bearing deletions of both genes encoding for CK2 regulatory subunits, shows a delay of S-phase entrance, due to a severe reduction of the expression of SBF- and MBF-dependent genes. This transcriptional defect is caused by an impaired recruitment of Swi6 and Swi4 to G1-gene promoters. Moreover, CK2 α and β' subunits interact with the RNA polymerase II, whose binding to G1-promoters is positively regulated by the CK2 holoenzyme. Collectively, these findings suggest a novel role for the CK2 holoenzyme in the activation of G1-transcription.

Date: Jul. 19, 2013

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