Interactions of protein kinase CK2beta subunit within the holoenzyme and with other proteins.
Protein kinase CK2 is a ubiquitous, highly conserved protein kinase with a tetrameric alpha2beta2 structure. For the formation of this tetrameric complex a beta-alpha dimer seems to be a prerequisite. Using the two-hybrid system and a series of CK2beta deletion mutants, we mapped domains involved in alpha-beta and beta-beta interactions. ... We also detected an intramolecular beta interaction within the amino acid stretch 132-165. Using CK2beta as a bait in a two-hybrid library screening several new putative cellular partners have been identified, among them the S6 kinase p90rsk, the putative tumor suppressor protein Doc-1, the Fas-associated protein FAF1, the mitochondrial translational initiation factor 2 and propionyl CoA carboxylase beta subunit.
Mesh Terms:
Casein Kinase II, Holoenzymes, Humans, Protein Binding, Protein-Serine-Threonine Kinases, Recombinant Proteins
Casein Kinase II, Holoenzymes, Humans, Protein Binding, Protein-Serine-Threonine Kinases, Recombinant Proteins
Mol. Cell. Biochem.
Date: Jan. 01, 1999
PubMed ID: 10094392
View in: Pubmed Google Scholar
Download Curated Data For This Publication
1573
Switch View:
- Interactions 2