Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID.
Our characterization of the Saccharomyces cerevisiae TATA box-binding protein (TBP) has led to the identification of nine specific yeast TBP-associated factors (TAFs) ranging in size from 170 to 25 kDa. The amino acid sequence derived from a purified TAF with an apparent M(r) of 170,000 indicates that yeast Taf170 is ... encoded by the essential yeast gene MOT1. We describe in this report a series of experiments that demonstrate that the protein encoded by MOT1 is a bona fide yeast TAF and that Taf170 forms a separate complex with TBP distinct from the RNA polymerase II-specific multisubunit transcription factor IID TBP-TAF complex. The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed.
Mesh Terms:
Adenosine Triphosphatases, Amino Acid Sequence, Base Sequence, Blotting, Western, Cloning, Molecular, DNA Helicases, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Oligodeoxyribonucleotides, Precipitin Tests, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA Box, TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, Transcription Factor TFIID, Transcription Factors
Adenosine Triphosphatases, Amino Acid Sequence, Base Sequence, Blotting, Western, Cloning, Molecular, DNA Helicases, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Oligodeoxyribonucleotides, Precipitin Tests, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA Box, TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, Transcription Factor TFIID, Transcription Factors
J. Biol. Chem.
Date: Sep. 16, 1994
PubMed ID: 8083216
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