Breast cancer cells secreted platelet-derived growth factor-induced motility of vascular smooth muscle cells is mediated through neuropilin-1.
Motility of vascular smooth muscle cells (SMCs) is an essential step for both normal and pathologic angiogenesis. We report here that breast tumor cells, such as MCF-7 and MDA-MB-231, can modulate this SMC migration. We present evidence that the tumor cell-derived platelet-derived growth factor (PDGF) is the key regulator of ... vascular SMCs motility induced by breast cancer cells. PDGF significantly upregulates neuropilin-1 (NRP-1) mRNA expression and protein production in aortic smooth muscle cells (AOSMCs) and depletion of NRP-1 production by AOSMCs with specific short hairpin RNA (shRNA) prevents the PDGF-dependent migration of vascular SMCs. Moreover, we demonstrate that PDGF physically interacts with NRP-1. We propose that tumor-derived PDGF and NRP-1 of AOSMCs function as a relay system that promotes motility of vascular SMCs.
Mesh Terms:
Base Sequence, Breast Neoplasms, Cell Line, Tumor, Cell Movement, Cells, Cultured, Culture Media, Conditioned, DNA Primers, Humans, Muscle, Smooth, Vascular, Neuropilin-1, Platelet-Derived Growth Factor, Reverse Transcriptase Polymerase Chain Reaction
Base Sequence, Breast Neoplasms, Cell Line, Tumor, Cell Movement, Cells, Cultured, Culture Media, Conditioned, DNA Primers, Humans, Muscle, Smooth, Vascular, Neuropilin-1, Platelet-Derived Growth Factor, Reverse Transcriptase Polymerase Chain Reaction
Mol. Carcinog.
Date: Nov. 01, 2006
PubMed ID: 16847823
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