Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85.
Induction of the yeast gene PHO5 is mediated by the transcription factors PHO2 and PHO4. PHO5 transcription is not detectable in high phosphate; it is thought that the negative regulators PHO80 and PHO85 inactivate PHO2 and PHO4. Here it is reported that PHO80 has homology to yeast cyclins and interacts ... with PHO85, a p34cdc2/CDC28-related protein kinase. The PHO80-PHO85 complex phosphorylates PHO4; this phosphorylation is correlated with negative regulation of PHO5. These results demonstrate the existence of a cyclin-cdk complex that is used for a regulatory process other than cell-cycle control and identify a physiologically relevant substrate for this complex.
Mesh Terms:
Acid Phosphatase, Amino Acid Sequence, Culture Media, Cyclin-Dependent Kinases, Cyclins, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Models, Genetic, Molecular Sequence Data, Phosphates, Phosphorylation, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Acid Phosphatase, Amino Acid Sequence, Culture Media, Cyclin-Dependent Kinases, Cyclins, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Models, Genetic, Molecular Sequence Data, Phosphates, Phosphorylation, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Science
Date: Feb. 25, 1994
PubMed ID: 8108735
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