Regulation of the transcriptional activation of the androgen receptor by the UXT binding protein VHL.

Loss and/or inactivation of VHL (the von Hippel Lindau tumour suppressor) cause various tumours. Using a yeast two-hybrid system, we have identified an AR co-activator, UXT, as a VHL-interacting protein. GST pull-down and co-immunoprecipitation assays show that UXT interacts with VHL. In addition, UXT recruits VHL to the nucleus. VHL ...
associates with the DBD and hinge domains of the AR and induces AR ubiquitination. Moreover, VHL interaction with the AR activates AR transactivation upon DHT treatment. VHL knockdown inhibits AR ubiquitination and decreases transcriptional activation of the AR. Our data suggest that the VHL-UXT interaction and VHL-induced ubiquitination of AR regulate transcriptional activation of the AR.
Biochem. J.
Date: Aug. 21, 2013
Download Curated Data For This Publication
158017
Switch View:
  • Interactions 11
  • PTM Genes 1