Tyrosine phosphorylation and recruitment of ZAP-70 to the CD3-TCR complex are defective after CD2 stimulation.
CD2 has been described as an alternative transduction pathway sharing the same biochemical cascade as CD3. The T cell-specific protein tyrosine kinase (PTK) ZAP-70 is believed to play a key role in early tyrosine phosphorylations of cellular proteins induced by CD3 stimulation. We show in the present report that ZAP-70 ... is insignificantly tyrosine phosphorylated and recruited to CD3 after CD2 stimulation in Jurkat T cells. The same result is obtained for p72syk, a PTK structurally and functionally related to ZAP-70. Several studies have suggested a model in which p56lck would be responsible after CD3 triggering for the phosphorylation of particular tyrosine residues in the immunoreceptor tyrosine-based activation motifs of the CD3 chains, inducing the recruitment and the tyrosine phosphorylation of ZAP-70. In Jurkat cells, p56lck is required for CD3- or CD2-induced tyrosine phosphorylations and clearly activated after CD2 cross-linking. However, we find that the CD3 complex, and especially its zeta-chain, are faintly tyrosine phosphorylated after CD2 triggering. By contrast, CD2 induces PLCgamma-1 tyrosine phosphorylation as efficiently as CD3, with a correlated inositol phosphate production and intracellular calcium increase, and even a higher production of IL-2. Interestingly, the SH2 domains of PLCgamma-1 associate with ZAP-70 upon CD3 stimulation while they bind, in CD2-activated cells, to a heavily tyrosine-phosphorylated 62-kDa protein. Altogether, these findings suggest that CD2 could bypass the PTK ZAP-70 for PLCgamma-1 activation and involves a preferential cascade comprising p56lck and a p62 protein, possibly acting as an anchor molecule.
Mesh Terms:
Antigens, CD2, Enzyme Precursors, Humans, Intracellular Signaling Peptides and Proteins, Jurkat Cells, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Phosphorylation, Protein Binding, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-fyn, Receptor-CD3 Complex, Antigen, T-Cell, Signal Transduction, Type C Phospholipases, ZAP-70 Protein-Tyrosine Kinase, src Homology Domains, src-Family Kinases
Antigens, CD2, Enzyme Precursors, Humans, Intracellular Signaling Peptides and Proteins, Jurkat Cells, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Phosphorylation, Protein Binding, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-fyn, Receptor-CD3 Complex, Antigen, T-Cell, Signal Transduction, Type C Phospholipases, ZAP-70 Protein-Tyrosine Kinase, src Homology Domains, src-Family Kinases
J. Immunol.
Date: Nov. 15, 1996
PubMed ID: 8906806
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