The Rad52-Rad59 complex interacts with Rad51 and replication protein A.

The RAD52 gene is essential for homology-dependent repair of double-strand breaks in Saccharomyces cerevisiae. Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-Rad52-Rad59 complexes. The N-terminal region of Rad52, which is required for self-interaction to form ...
a ring structure, is required for interaction with Rad59. Rad59 also shows self-interaction suggesting the formation of heteromeric and homomeric rings of Rad52 and Rad59. In wild-type cells, we propose the Rad51-Rad52-Rad59 complex is involved in conservative recombination events, including gene conversion and reciprocal recombination, whereas the Rad52-Rad59 complex participates in single-strand annealing.
Mesh Terms:
Binding Sites, DNA, DNA Damage, DNA Repair, DNA Replication, DNA, Single-Stranded, DNA-Binding Proteins, Precipitin Tests, Protein Binding, Rad51 Recombinase, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, Replication Protein A, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
DNA Repair (Amst.)
Date: Oct. 07, 2003
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