Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage.
Our study first proposed that curcumin could protect human endothelial cells from the damage caused by oxidative stress via autophagy. Furthermore, our results revealed that curcumin causes some novel cellular mechanisms that promote autophagy as a protective effect. Pretreatment with curcumin remarkably improves the survival of human umbilical vein endothelial ... cells (HUVECs) from H 2O 2-induced viability loss, which specifically evokes an autophagic response. Exposed to H 2O 2, curcumin-treated HUVECs upregulate the level of microtubule-associated protein 1 light chain 3-II (LC3-II), the number of autophagosomes, and the degradation of p62. We show that this compound promotes BECN1 expression and inhibits the phosphatidylinositol 3-kinase (PtdIns3K)-AKT-mechanistic target of rapamycin (MTOR) signaling pathway. Curcumin can also reverse FOXO1 (a mediator of autophagy) nuclear localization along with causing an elevated level of cytoplasmic acetylation of FOXO1 and the interaction of acetylated FOXO1 and ATG7, under the circumstance of oxidative stress. Additionally, knockdown of FOXO1 by shRNA inhibits not only the protective effects that curcumin induced, but the autophagic process, from the quantity of LC3-II to the expression of RAB7. These results suggest that curcumin induces autophagy, indicating that curcumin has the potential for use as an autophagic-related antioxidant for prevention and treatment of oxidative stress. These data uncover a brand new protective mechanism involving FOXO1 as having a critical role in regulating autophagy in HUVECs, and suggest a novel role for curcumin in inducing a beneficial form of autophagy in HUVECs, which may be a potential multitargeted therapeutic avenue for the treatment of oxidative stress-related cardiovascular diseases.
Mesh Terms:
Acetylation, Apoptosis, Apoptosis Regulatory Proteins, Autophagy, Cell Nucleus, Cell Survival, Curcumin, Cytoprotection, Down-Regulation, Forkhead Transcription Factors, Gene Knockdown Techniques, Human Umbilical Vein Endothelial Cells, Humans, Hydrogen Peroxide, Membrane Proteins, Models, Biological, Oxidative Stress, Phosphatidylinositol 3-Kinases, Protective Agents, Protein Binding, Protein Transport, Proto-Oncogene Proteins c-akt, Proto-Oncogene Proteins c-bcl-2, Signal Transduction, TOR Serine-Threonine Kinases, Ubiquitin-Activating Enzymes, Up-Regulation, rab GTP-Binding Proteins
Acetylation, Apoptosis, Apoptosis Regulatory Proteins, Autophagy, Cell Nucleus, Cell Survival, Curcumin, Cytoprotection, Down-Regulation, Forkhead Transcription Factors, Gene Knockdown Techniques, Human Umbilical Vein Endothelial Cells, Humans, Hydrogen Peroxide, Membrane Proteins, Models, Biological, Oxidative Stress, Phosphatidylinositol 3-Kinases, Protective Agents, Protein Binding, Protein Transport, Proto-Oncogene Proteins c-akt, Proto-Oncogene Proteins c-bcl-2, Signal Transduction, TOR Serine-Threonine Kinases, Ubiquitin-Activating Enzymes, Up-Regulation, rab GTP-Binding Proteins
Autophagy
Date: May. 01, 2012
PubMed ID: 22622204
View in: Pubmed Google Scholar
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