Ordering the final events in yeast exocytosis.
In yeast, assembly of exocytic soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor (SNARE) complexes between the secretory vesicle SNARE Sncp and the plasma membrane SNAREs Ssop and Sec9p occurs at a late stage of the exocytic reaction. Mutations that block either secretory vesicle delivery or tethering prevent SNARE complex ... assembly and the localization of Sec1p, a SNARE complex binding protein, to sites of secretion. By contrast, wild-type levels of SNARE complexes persist in the sec1-1 mutant after a secretory block is imposed, suggesting a role for Sec1p after SNARE complex assembly. In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane.
Mesh Terms:
Adenosine Triphosphatases, Carrier Proteins, Cell Membrane, Cell Polarity, Exocytosis, Fungal Proteins, GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Membrane Fusion, Membrane Proteins, Models, Biological, Munc18 Proteins, N-Ethylmaleimide-Sensitive Proteins, Nerve Tissue Proteins, Qa-SNARE Proteins, R-SNARE Proteins, SNARE Proteins, Saccharomyces cerevisiae Proteins, Transport Vesicles, Vesicular Transport Proteins, Yeasts
Adenosine Triphosphatases, Carrier Proteins, Cell Membrane, Cell Polarity, Exocytosis, Fungal Proteins, GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Membrane Fusion, Membrane Proteins, Models, Biological, Munc18 Proteins, N-Ethylmaleimide-Sensitive Proteins, Nerve Tissue Proteins, Qa-SNARE Proteins, R-SNARE Proteins, SNARE Proteins, Saccharomyces cerevisiae Proteins, Transport Vesicles, Vesicular Transport Proteins, Yeasts
J. Cell Biol.
Date: Oct. 16, 2000
PubMed ID: 11038189
View in: Pubmed Google Scholar
Download Curated Data For This Publication
15874
Switch View:
- Interactions 4