Ras GTPase-activating protein-associated p62 is a major v-Src-SH3-binding protein.

Oncogenic transformation by v-Src is accompanied by marked morphological changes and cytoskeletal reorganization. Yet, the cytoskeleton-associated proteins with which v-Src interacts are largely unknown. We have studied the binding of v-Src-SH3 domain to cellular proteins utilizing a blot overlay procedure with a GST-v-Src-SH3 fusion protein as probe. A major 62-64 ...
kDa v-Src-SH3-binding protein, present in detergent-insoluble cellular fractions, was identified as p21ras-GTPase-activating protein-associated p62 (GAPA62). In non-transformed cells, including NIH 3T3 cells, GAPA62 was present in both the RIP A-soluble and RIP A-insoluble fractions, but only the latter form was tyrosine-phosphorylated. In contrast, in polyoma middle T antigen-transformed 3T3 cells, GAPA62 was present only in the RIP A-insoluble fraction, where it was highly phosphorylated. It is suggested that tyrosine phosphorylation of GAPA62 may be an important determinant of its cellular localization and its possible function as a mediator of v-Src actions.
Mesh Terms:
3T3 Cells, Animals, DNA-Binding Proteins, Detergents, Glutathione Transferase, Humans, Immunoblotting, Mice, Phosphoproteins, Phosphorylation, Phosphotyrosine, Precipitin Tests, RNA-Binding Proteins, Recombinant Fusion Proteins, Solubility, Tyrosine, src Homology Domains, src-Family Kinases
FEBS Lett.
Date: Feb. 10, 1997
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