The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins.
The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, ... including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.
Mesh Terms:
Adaptor Proteins, Vesicular Transport, COP-Coated Vesicles, Carrier Proteins, Coat Protein Complex I, Golgi Apparatus, Macromolecular Substances, Membrane Proteins, Mutation, Peptides, Protein Binding, Protein Transport, SNARE Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, rab GTP-Binding Proteins
Adaptor Proteins, Vesicular Transport, COP-Coated Vesicles, Carrier Proteins, Coat Protein Complex I, Golgi Apparatus, Macromolecular Substances, Membrane Proteins, Mutation, Peptides, Protein Binding, Protein Transport, SNARE Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, rab GTP-Binding Proteins
J. Cell Biol.
Date: May. 13, 2002
PubMed ID: 12011112
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