Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells.
The genome of Saccharomyces cerevisia encodes four mismatch repair MutL proteins and these proteins form three heterocomplexes: Mlh1-Mlh2, Mlh1-Mlh3, and Mlh1-Pms1. Only, the Mlh1-Mlh3 heterocomplex has been implicated specifically in promotion of meiotic crossing-over. In this report, we show that yeast Mlh3 co-immunoprecipitates with Sgs1 helicase in sporulating cells at ... late stage of meiotic prophase I. Sgs1, a member of the RecQ DNA helicase family, appears to form a stable complex with topoisomerase III (Top3) during meiosis. We suggest that Mlh1-Mlh3 heterocomplex may act as a molecular matchmaker to coordinate Sgs1-Top3 complex in the resolution of meiotic recombination intermediates.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Blotting, Western, Carrier Proteins, DNA Helicases, DNA Topoisomerases, Type I, Fungal Proteins, Meiosis, Polymerase Chain Reaction, Precipitin Tests, Prophase, Protein Binding, RecQ Helicases, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors
Adaptor Proteins, Signal Transducing, Blotting, Western, Carrier Proteins, DNA Helicases, DNA Topoisomerases, Type I, Fungal Proteins, Meiosis, Polymerase Chain Reaction, Precipitin Tests, Prophase, Protein Binding, RecQ Helicases, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors
Biochem. Biophys. Res. Commun.
Date: Aug. 30, 2002
PubMed ID: 12200140
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