Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p.
The mating response pathway of the yeast Saccharomyces cerevisiae includes a heterotrimeric guanine nucleotide-binding protein (G protein) that activates a mitogen-activated protein MAP kinase cascade by an unknown mechanism. An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified. A haploid-specific ... interaction between the amino terminus of Ste5p and the G protein beta subunit Ste4p was also detected in a two-hybrid assay, and the product of a signaling-defective allele of STE4 was defective in this interaction. In cells with a constitutively activated pheromone response pathway, epitope-tagged Ste4p was coimmunoprecipitated with Ste5p. Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Base Sequence, Calcium-Calmodulin-Dependent Protein Kinases, Carrier Proteins, Cell Division, Fungal Proteins, GTP-Binding Protein beta Subunits, GTP-Binding Protein gamma Subunits, GTP-Binding Proteins, Heterotrimeric GTP-Binding Proteins, Molecular Sequence Data, Mutation, Pheromones, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Transformation, Genetic
Adaptor Proteins, Signal Transducing, Base Sequence, Calcium-Calmodulin-Dependent Protein Kinases, Carrier Proteins, Cell Division, Fungal Proteins, GTP-Binding Protein beta Subunits, GTP-Binding Protein gamma Subunits, GTP-Binding Proteins, Heterotrimeric GTP-Binding Proteins, Molecular Sequence Data, Mutation, Pheromones, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Transformation, Genetic
Science
Date: Sep. 15, 1995
PubMed ID: 7667635
View in: Pubmed Google Scholar
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