ATM-related Tel1 associates with double-strand breaks through an Xrs2-dependent mechanism.
In budding yeast, TEL1 encodes a protein closely related to ATM. Xrs2 is an Nbs1 homolog and forms a complex with Mre11 and Rad50. We show here that Tel1 associates with double-strand breaks (DSBs) through a mechanism dependent on the C terminus of Xrs2. Although Xrs2 is required for the ... DNA degradation at DSBs, the C-terminal Xrs2 truncation does not affect the degradation. Tel1 and the C terminus of Xrs2 are similarly involved in cell survival and Rad53 phosphorylation after DNA damage. Our findings suggest that the Tel1 association with DNA lesions is required for the activation of DNA damage responses.
Mesh Terms:
Cell Cycle Proteins, Cell Survival, DNA Damage, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Intracellular Signaling Peptides and Proteins, Phosphorylation, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Tumor Suppressor Proteins
Cell Cycle Proteins, Cell Survival, DNA Damage, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Intracellular Signaling Peptides and Proteins, Phosphorylation, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Tumor Suppressor Proteins
Genes Dev.
Date: Aug. 15, 2003
PubMed ID: 12923051
View in: Pubmed Google Scholar
Download Curated Data For This Publication
15970
Switch View:
- Interactions 6