A universal expression tag for structural and functional studies of proteins.
Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR ... domains with autophagy modifiers.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Expressed Sequence Tags, Genetic Vectors, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Protein Biosynthesis, Protein Structure, Tertiary, Proteins, Recombinant Fusion Proteins, Ubiquitin
Amino Acid Sequence, Base Sequence, Expressed Sequence Tags, Genetic Vectors, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Protein Biosynthesis, Protein Structure, Tertiary, Proteins, Recombinant Fusion Proteins, Ubiquitin
Chembiochem
Date: May. 07, 2012
PubMed ID: 22434781
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