Evidence for cyclooxygenase-1 association with caveolin-1 and -2 in cultured human embryonic kidney (HEK 293) cells.
The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high levels of caveolin-2 and low level of ... caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Caveolae rich membranous fractions from the HEK 293 cells contained both COX-1 and caveolin-1 or caveolin-2 in same fractions. The experiments of immuno-precipitation showed complex formation between the COX-1 and caveolin-1 or caveolin-2 in the HEK 293 cells. Confocal microscopic results also support co-localization of COX-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with cylooxygenase-1 in caveolae suggested that caveolin would play an important role in regulating the function of COX-1.
Mesh Terms:
Blotting, Western, Caveolin 1, Caveolin 2, Caveolins, Cell Membrane, Cells, Cultured, Cyclooxygenase 1, DNA Primers, Humans, Immunoprecipitation, Isoenzymes, Membrane Proteins, Microscopy, Confocal, Prostaglandin-Endoperoxide Synthases, Reverse Transcriptase Polymerase Chain Reaction
Blotting, Western, Caveolin 1, Caveolin 2, Caveolins, Cell Membrane, Cells, Cultured, Cyclooxygenase 1, DNA Primers, Humans, Immunoprecipitation, Isoenzymes, Membrane Proteins, Microscopy, Confocal, Prostaglandin-Endoperoxide Synthases, Reverse Transcriptase Polymerase Chain Reaction
IUBMB Life
Date: Apr. 01, 2004
PubMed ID: 15230350
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