The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae.
We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with ... snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1 p is important in the optimization of splicing.
Mesh Terms:
Amino Acid Sequence, Cloning, Molecular, Fungal Proteins, Gene Deletion, Genes, Reporter, Molecular Sequence Data, Mutation, Nuclear Proteins, Precipitin Tests, RNA Precursors, RNA Splicing, RNA, Messenger, RNA-Binding Proteins, Ribonucleoproteins, Small Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Spliceosomes
Amino Acid Sequence, Cloning, Molecular, Fungal Proteins, Gene Deletion, Genes, Reporter, Molecular Sequence Data, Mutation, Nuclear Proteins, Precipitin Tests, RNA Precursors, RNA Splicing, RNA, Messenger, RNA-Binding Proteins, Ribonucleoproteins, Small Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Spliceosomes
RNA
Date: Mar. 01, 1999
PubMed ID: 10094305
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