Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins.
The regulation of membrane traffic involves the Rab family of Ras-related GTPases, of which there are a total of 11 members in the yeast Saccharomyces cerevisiae. Previous work has identified PRA1 as a dual prenylated Rab GTPase and VAMP2 interacting protein [Martinic et al. (1999) J. Biol. Chem. 272, 26991-26998]. ... In this study we demonstrate that the yeast counterpart of PRA1 interacts with Rab proteins and with Yip1p, a membrane protein of unknown function that has been reported to interact specifically with the Rab proteins Ypt1p and Ypt31p. Yeast Pra1p/Yip3p is a factor capable of biochemical interaction with a panel of different Rab proteins and does not show in vitro specificity for any particular Rab. The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation.
Mesh Terms:
Blotting, Western, Electrophoresis, Polyacrylamide Gel, Fungal Proteins, Membrane Proteins, Protein Binding, Receptors, Cell Surface, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Two-Hybrid System Techniques, Vesicular Transport Proteins, rab GTP-Binding Proteins, rab1 GTP-Binding Proteins, rab5 GTP-Binding Proteins
Blotting, Western, Electrophoresis, Polyacrylamide Gel, Fungal Proteins, Membrane Proteins, Protein Binding, Receptors, Cell Surface, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Two-Hybrid System Techniques, Vesicular Transport Proteins, rab GTP-Binding Proteins, rab1 GTP-Binding Proteins, rab5 GTP-Binding Proteins
Biochem. Biophys. Res. Commun.
Date: Jan. 18, 2002
PubMed ID: 11785952
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