SUMO-1 protease-1 regulates gene transcription through PML.
During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered ... the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cell Nucleus Structures, Cysteine Endopeptidases, Hela Cells, Humans, Mice, Molecular Sequence Data, Mutation, Neoplasm Proteins, Nuclear Proteins, Phosphorylation, Promoter Regions, Genetic, Protein Binding, Proto-Oncogene Proteins c-jun, Sequence Homology, Nucleic Acid, Substrate Specificity, Transcription Factors, Transcriptional Activation, Tumor Suppressor Proteins
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cell Nucleus Structures, Cysteine Endopeptidases, Hela Cells, Humans, Mice, Molecular Sequence Data, Mutation, Neoplasm Proteins, Nuclear Proteins, Phosphorylation, Promoter Regions, Genetic, Protein Binding, Proto-Oncogene Proteins c-jun, Sequence Homology, Nucleic Acid, Substrate Specificity, Transcription Factors, Transcriptional Activation, Tumor Suppressor Proteins
Mol. Cell
Date: Oct. 01, 2002
PubMed ID: 12419228
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