Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays.
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required ... an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.
Mesh Terms:
CCAAT-Binding Factor, Cell Cycle Proteins, Chromatin, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Herpes Simplex Virus Protein Vmw65, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Kinases, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcriptional Activation, Yeasts
CCAAT-Binding Factor, Cell Cycle Proteins, Chromatin, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Herpes Simplex Virus Protein Vmw65, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Kinases, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcriptional Activation, Yeasts
Mol. Cell
Date: Oct. 01, 1999
PubMed ID: 10549297
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