Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator.
Mutations in three subunits of the SWI/SNF complex and in the Med2p subunit of the SRB/mediator of pol II holoenzyme impaired Gcn4p-activated transcription of HIS3 without reducing Gcn4p-independent transcription of this gene. Recombinant Gcn4p interacted with SWI/SNF and SRB/mediator subunits in cell extracts in a manner dependent on the same ... hydrophobic clusters in the Gcn4p activation domain; however, higher concentrations of Gcn4p were required for binding to SWI/SNF versus SRB/mediator subunits. In addition, SRB/mediator and SWI/SNF subunits did not coimmunopreciptate from the extracts. These findings, together with the fact that Gcn4p specifically interacted with purified SWI/SNF, strongly suggest that Gcn4p independently recruits SWI/SNF and holoenzyme to its target promoters in the course of activating transcription.
Mesh Terms:
DNA Polymerase II, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Hydro-Lyases, Mediator Complex, Mutation, Nuclear Proteins, Nucleosomes, Protein Binding, Protein Kinases, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcriptional Activation, Yeasts
DNA Polymerase II, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Hydro-Lyases, Mediator Complex, Mutation, Nuclear Proteins, Nucleosomes, Protein Binding, Protein Kinases, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcriptional Activation, Yeasts
Mol. Cell
Date: Oct. 01, 1999
PubMed ID: 10549298
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