The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress.
The anaphase-promoting complex or cyclosome (APC/C) is a multi-subunit ubiquitin ligase that regulates exit from mitosis and G1 phase of the cell cycle. Although the regulation and function of APC/C(Cdh1) in the unperturbed cell cycle is well studied, little is known of its role in non-genotoxic stress responses. Here, we ... demonstrate the role of APC/C(Cdh1) (APC/C activated by Cdh1 protein) in cellular protection from endoplasmic reticulum (ER) stress. Activation of APC/C(Cdh1) under ER stress conditions is evidenced by Cdh1-dependent degradation of its substrates. Importantly, the activity of APC/C(Cdh1) maintains the ER stress checkpoint, as depletion of Cdh1 by RNAi impairs cell cycle arrest and accelerates cell death following ER stress. Our findings identify APC/C(Cdh1) as a regulator of cell cycle checkpoint and cell survival in response to proteotoxic insults.
Mesh Terms:
Anaphase-Promoting Complex-Cyclosome, Cadherins, Cell Cycle Checkpoints, Cell Survival, Endoplasmic Reticulum Stress, G1 Phase, HeLa Cells, Humans, Mitosis, RNA Interference, RNA, Small Interfering, Ubiquitin-Protein Ligase Complexes
Anaphase-Promoting Complex-Cyclosome, Cadherins, Cell Cycle Checkpoints, Cell Survival, Endoplasmic Reticulum Stress, G1 Phase, HeLa Cells, Humans, Mitosis, RNA Interference, RNA, Small Interfering, Ubiquitin-Protein Ligase Complexes
PLoS ONE
Date: Apr. 28, 2012
PubMed ID: 22539978
View in: Pubmed Google Scholar
Download Curated Data For This Publication
162317
Switch View:
- Interactions 7