SAC3 may link nuclear protein export to cell cycle progression.
Selective movement of proteins between the nucleus and the cytoplasm is a regulatory mechanism exploited extensively by the eukaryotic cell. We have identified the evolutionarily conserved Sac3 protein, which was implicated previously in the regulation of mitosis [Bauer, A. & Koelling, R. (1996) J. Cell Sci. 109, 1575-1583] as a ... novel mediator of nuclear protein export. We show that Sac3p is localized to the nuclear pore, where it interacts with nucleoporins. Loss of SAC3 function results in a block in nuclear export of a nuclear export signal-containing reporter protein. Our results also demonstrate that SAC3 interacts genetically with the nuclear protein export factors Crm1p/Xpo1p and Yrb2p. Taken together, these data indicate a link between nuclear protein export and transition through the cell cycle.
Mesh Terms:
Amino Acid Sequence, Biological Transport, Calcium-Binding Proteins, Cell Cycle, Fluorescent Antibody Technique, Indirect, Fungal Proteins, Gene Deletion, Green Fluorescent Proteins, Luminescent Proteins, Molecular Sequence Data, Nuclear Pore Complex Proteins, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Porins, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid
Amino Acid Sequence, Biological Transport, Calcium-Binding Proteins, Cell Cycle, Fluorescent Antibody Technique, Indirect, Fungal Proteins, Gene Deletion, Green Fluorescent Proteins, Luminescent Proteins, Molecular Sequence Data, Nuclear Pore Complex Proteins, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Porins, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid
Proc. Natl. Acad. Sci. U.S.A.
Date: Mar. 28, 2000
PubMed ID: 10716708
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