The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p.
There are several kinases in Saccharomyces cerevisiae that phosphorylate the CTD of RNA polymerase II, but specific and distinct functions of the phospho-CTDs generated by the different kinases are not well understood. A genetic screen for suppressors of loss of yeast CTD kinase I (CTDK-I) function (by deletion of the ... catalytic subunit gene CTK1) identified PTI1, a potential 3' cleavage/polyadenylation factor. Genetic and physical interactions connect Pti1p to components of CF IA and CF II/CPF, and mutations of PTI1 or CTK1 affect 3' cleavage site choice and transcript abundance of particular genes. Therefore, one important function of the CTDK-I-generated phospho-CTD appears to be the coupling of transcription to 3' processing of pre-mRNAs by a Pti1p-containing complex.
Mesh Terms:
Amino Acid Sequence, Genes, Essential, Genes, Fungal, Molecular Sequence Data, Peptide Fragments, Poly A, Protein Kinases, Protein Subunits, Protein-Serine-Threonine Kinases, RNA Polymerase II, RNA Precursors, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Substrate Specificity, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Amino Acid Sequence, Genes, Essential, Genes, Fungal, Molecular Sequence Data, Peptide Fragments, Poly A, Protein Kinases, Protein Subunits, Protein-Serine-Threonine Kinases, RNA Polymerase II, RNA Precursors, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Substrate Specificity, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Mol. Cell
Date: Dec. 01, 2002
PubMed ID: 12504017
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