Structural studies of a yeast quaternary transcription-initiation complex.
A 96.7 kDa quaternary transcription-factor complex consisting of the conserved core domains of yeast TBP, TFIIA, TFIIB and TATA-box DNA has been assembled from purified components. Crystals of the complex were obtained by the hanging-drop vapor-diffusion method. Native data sets were collected at synchrotron sources. Crystal form I belongs to ... space group R32, with unit-cell parameters a = b = 173.2, c = 164.1 A, and diffracts to 2.5 resolution, but contains substoichiometric amounts of TFIIB. Crystal form II was assembled with a longer piece of DNA than that used for form I crystals and contains the complete quaternary complex. These crystals belong to space group P42(1)2, with unit-cell parameters a = b = 141.7, c = 112.0 A, and diffract to 3.6 A resolution.
Mesh Terms:
Crystallization, Crystallography, X-Ray, DNA, DNA-Binding Proteins, Nucleic Acid Conformation, Protein Conformation, Saccharomyces cerevisiae, TATA Box, TATA-Box Binding Protein, Transcription Factor TFIIA, Transcription Factor TFIIB, Transcription Factors
Crystallization, Crystallography, X-Ray, DNA, DNA-Binding Proteins, Nucleic Acid Conformation, Protein Conformation, Saccharomyces cerevisiae, TATA Box, TATA-Box Binding Protein, Transcription Factor TFIIA, Transcription Factor TFIIB, Transcription Factors
Acta Crystallogr. D Biol. Crystallogr.
Date: Mar. 01, 2001
PubMed ID: 11223526
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