Identification of a brain-specific APC homologue, APCL, and its interaction with beta-catenin.
We isolated a novel gene, APCL, that showed significant homology to the adenomatous polyposis coli (APC) tumor suppressor gene. This novel gene, located on chromosome 19p13.3, encodes a protein of 2303 amino acids that is expressed specifically in the brain. The predicted protein of APCL contains five copies of a ... 20-amino-acid motif (FXVEXTPXCFSRXSSLSSLS). Like APC, this domain of APCL was able to bind to beta-catenin and deplete the intracellular beta-catenin pool. A reporter-gene assay revealed that APCL could also regulate interaction of beta-catenin with T cell-specific transcription factor, although less actively than APC. These results suggest that the APCL protein may be involved in the Wnt/Wingless signal pathway, and the identification of a novel relative of APC may provide new insights into the function of APC.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Brain, Chromosome Mapping, Chromosomes, Human, Pair 19, Colonic Neoplasms, Cytoskeletal Proteins, Genes, APC, Genes, Tumor Suppressor, Humans, Molecular Sequence Data, Organ Specificity, Trans-Activators, Tumor Cells, Cultured, beta Catenin
Amino Acid Sequence, Base Sequence, Brain, Chromosome Mapping, Chromosomes, Human, Pair 19, Colonic Neoplasms, Cytoskeletal Proteins, Genes, APC, Genes, Tumor Suppressor, Humans, Molecular Sequence Data, Organ Specificity, Trans-Activators, Tumor Cells, Cultured, beta Catenin
Cancer Res.
Date: Nov. 15, 1998
PubMed ID: 9823329
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