Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast.
We previously isolated the SKN7 gene in a screen designed to isolate new components of the G1-S cell cycle transcription machinery in budding yeast. We have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several genetic interactions. Skn7 ... overexpression is lethal and is suppressed by a mutation in MBP1. Similarly, high overexpression of Mbp1 is lethal and can be suppressed by skn7 mutations. SKN7 is also required for MBP1 function in a mutant compromised for G1-specific transcription. Gel-retardation assays indicate that Skn7 is not an integral part of MBF. However, a physical interaction between Skn7 and Mbp1 was detected using two-hybrid assays and GST pulldowns. Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process.
Mesh Terms:
Cell Cycle, Cell Survival, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Mutation, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transformation, Genetic
Cell Cycle, Cell Survival, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Mutation, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transformation, Genetic
Mol. Biol. Cell
Date: Oct. 01, 1999
PubMed ID: 10512874
View in: Pubmed Google Scholar
Download Curated Data For This Publication
16399
Switch View:
- Interactions 16