Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2.
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
Mesh Terms:
Animals, Apoptosis, Carnitine O-Palmitoyltransferase, Intracellular Membranes, Membrane Proteins, Mice, Mitochondria, Precipitin Tests, Protein Binding, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Structure-Activity Relationship
Animals, Apoptosis, Carnitine O-Palmitoyltransferase, Intracellular Membranes, Membrane Proteins, Mice, Mitochondria, Precipitin Tests, Protein Binding, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Structure-Activity Relationship
Biochem. Biophys. Res. Commun.
Date: Feb. 24, 1997
PubMed ID: 9070836
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