Abruzzi KC, Magendantz M, Solomon F

Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Cells expressing a mutant allele of alpha-tubulin, tub1-729, are cold sensitive and arrest as large-budded cells with microtubule defects. The cold sensitivity of tub1-729 is suppressed by extra copies of a subset of the mitotic checkpoint genes BUB1, BUB3, and MPS1, but not MAD1, MAD2, and MAD3. This suppression by checkpoint genes does not depend upon their role in the MAD2-dependent spindle assembly checkpoint. In addition, BUB1 requires an intact kinase domain as well as Bub3p to suppress tub1-729. The data suggest that tub1-729 cells are defective in microtubule-kinetochore attachments and that the products of specific checkpoint genes can act either directly or indirectly to affect these attachments.

Mesh Terms:
Alleles, Base Sequence, Chromosomes, Fungal, DNA Primers, Genes, Fungal, Genotype, Mitosis, Mitotic Spindle Apparatus, Plasmids, Polymerase Chain Reaction, Saccharomyces cerevisiae, Temperature, Tubulin

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