Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons.

Analysis of the arc35-1 mutant has revealed previously that this component of the Arp2/3 complex is involved in organization of the actin cytoskeleton. Further characterization uncovered a cell division cycle phenotype with arrest as large-budded cells. Cells with correctly positioned metaphase spindles accumulated at the restrictive temperature. The observed metaphase ...
arrest most likely occurs by activation of the spindle assembly checkpoint, because arc35-1 was synthetically lethal with a deletion of BUB2. Arc35p activity is required late in G(1) for its cell cycle function. Both the actin and microtubule defects of arc35-1 can be suppressed by overexpression of calmodulin. Analysis of a collection of ts cmd1 mutants for their ability to suppress the actin and/or microtubule defect revealed that the two defects observed in arc35-1 are genetically separable. These data suggest that the actin defect is probably not the cause of the microtubule defect.
Mesh Terms:
Actin-Related Protein 2, Actin-Related Protein 3, Actins, Alleles, Bicyclo Compounds, Heterocyclic, Calmodulin, Cell Cycle Proteins, Cell Division, Cytoskeletal Proteins, Cytoskeleton, Fungal Proteins, G1 Phase, Gene Expression Regulation, Fungal, Macromolecular Substances, Metaphase, Microtubules, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Thiazoles, Thiazolidines, Tubulin
J. Cell. Sci.
Date: Feb. 01, 2000
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