Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint.

DPB11, a gene that suppresses mutations in two essential subunits of Saccharomyces cerevisiae DNA polymerase II(epsilon) encoded by POL2 and DPB2, was isolated on a multicopy plasmid. The nucleotide sequence of the DPB11 gene revealed an open reading frame predicting an 87-kDa protein. This protein is homologous to the Schizosaccharomyces ...
pombe rad4+/cut5+ gene product that has a cell cycle checkpoint function. Disruption of DPB11 is lethal, indicating that DPB11 is essential for cell proliferation. In thermosensitive dpb11-1 mutant cells, S-phase progression is defective at the nonpermissive temperature, followed by cell division with unequal chromosomal segregation accompanied by loss of viability.dpb11-1 is synthetic lethal with any one of the dpb2-1, pol2-11, and pol2-18 mutations at all temperatures. Moreover, dpb11 cells are sensitive to hydroxyurea, methyl methanesulfonate, and UV irradiation. These results strongly suggest that Dpb11 is a part of the DNA polymerase II complex during chromosomal DNA replication and also acts in a checkpoint pathway during the S phase of the cell cycle to sense stalled DNA replication.
Mesh Terms:
Amino Acid Sequence, Cell Cycle, Cell Cycle Proteins, DNA Polymerase II, DNA-Binding Proteins, Dose-Response Relationship, Radiation, Flow Cytometry, Fungal Proteins, Gene Dosage, Genes, Fungal, Genes, Suppressor, Hydroxyurea, Methyl Methanesulfonate, Molecular Sequence Data, Mutation, Phenotype, Protein Binding, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Transglutaminases, Ultraviolet Rays
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 05, 1995
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