Border control--a membrane-linked interactome of Arabidopsis.
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions ... such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Cell Membrane, Membrane Proteins, Protein Interaction Maps, Signal Transduction, Two-Hybrid System Techniques
Arabidopsis, Arabidopsis Proteins, Cell Membrane, Membrane Proteins, Protein Interaction Maps, Signal Transduction, Two-Hybrid System Techniques
Science
Date: May. 16, 2014
PubMed ID: 24833385
View in: Pubmed Google Scholar
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- Interactions 12,097