Functional interaction of beta-catenin with the transcription factor LEF-1.

The cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton, and they participate in the wnt/wingless signal pathway. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with ...
beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend. Microinjection of LEF-1 into XenoPus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus.
Mesh Terms:
Animals, Cadherins, Cell Line, Cell Nucleus, Cloning, Molecular, Cytoskeletal Proteins, DNA, DNA-Binding Proteins, Drosophila, Escherichia coli, Lymphoid Enhancer-Binding Factor 1, Nucleic Acid Conformation, Protein Binding, RNA, Messenger, Recombinant Proteins, Saccharomyces cerevisiae, Trans-Activators, Transcription Factors, Xenopus, Xenopus Proteins, beta Catenin
Nature
Date: Aug. 15, 1996
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