Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae.
In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2, a temperature-sensitive mutant of the Saccharomyces cerevisiae RCC1 homologue. One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being ... designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S. cerevisiae Ran/TC4 homologue Gsp1p, previously reported to suppress prp20-1, but not mtr1-2 or srm1-1. Furthermore, gtr1-11 suppressed the rna1-1, temperature-sensitive mutant of the Gsp1p GTPase-activating protein, but not the srp1-31, temperature-sensitive mutant of the S. cerevisiae importin alpha homologue. mtr1-2, srm1-1 and prp20-1 were also suppressed by overexpression of the mutated Gtr1p, Gtr1-11p. In summary, Gtr1p that was localized in the cytoplasm by immunofluoresence staining was suggested to function as a negative regulator for the Ran/TC4 GTPase cycle.
Mesh Terms:
Base Sequence, Cell Cycle Proteins, DNA Primers, DNA-Binding Proteins, Fungal Proteins, GTP Phosphohydrolases, Gene Deletion, Genes, Fungal, Genes, Suppressor, Guanine Nucleotide Exchange Factors, Mutation, Nuclear Proteins, Saccharomyces cerevisiae, Temperature, ran GTP-Binding Protein
Base Sequence, Cell Cycle Proteins, DNA Primers, DNA-Binding Proteins, Fungal Proteins, GTP Phosphohydrolases, Gene Deletion, Genes, Fungal, Genes, Suppressor, Guanine Nucleotide Exchange Factors, Mutation, Nuclear Proteins, Saccharomyces cerevisiae, Temperature, ran GTP-Binding Protein
J. Cell. Sci.
Date: Sep. 01, 1996
PubMed ID: 8886981
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